High expression of L-selectin ligand in secretory endometrium is associated with better endometrial receptivity and facilitates embryo implantation in human being

Am J Reprod Immunol. 2008 Aug;60(2):127-34. doi: 10.1111/j.1600-0897.2008.00604.x.


Problem: L-selectin ligand has displayed mediating adhesion at the maternal-fetal interface. Therefore, we investigated the impact of L-selectin ligand on establishing pregnancy in women undergoing in vitro fertilization and embryo transfer (IVF-ET).

Method of study: Endometrium between cycle days LH +6 to +9 was obtained from 56 Chinese women referred for IVF and tested for L-selectin ligand by immunohistochemistry and Western blot. The standard gonadotropin-releasing hormone agonist long protocol was used for ovarian stimulation.

Results: L-selectin ligand was localized in the endometrial gland and luminal epithelial cells. Western blot analysis of endometrium identified four bands and levels of component 1, 2 and 4 were significantly higher in the pregnancy group than in the non-pregnancy group (P < 0.05). Clinical pregnancy and implantation rates were higher in patients with high level L-selectin ligand compared with those with low level (53.6%versus 25.0%, and 27.1%versus 12.1%, respectively, P < 0.05).

Conclusion: The presence of higher level L-selectin ligand was associated with a better pregnancy outcome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Embryo Implantation / physiology*
  • Endometrium / cytology
  • Endometrium / metabolism*
  • Endometrium / physiology
  • Epithelial Cells / metabolism
  • Female
  • Fertilization in Vitro
  • Gonadotropin-Releasing Hormone / metabolism
  • Humans
  • L-Selectin / metabolism*
  • Ligands
  • Ovulation Induction
  • Pregnancy


  • Ligands
  • L-Selectin
  • Gonadotropin-Releasing Hormone