Development and validation of a mismatch amplification mutation PCR assay to monitor the dissemination of an emerging variant of Vibrio cholerae O1 biotype El Tor

Microbiol Immunol. 2008 Jun;52(6):314-7. doi: 10.1111/j.1348-0421.2008.00041.x.


A mismatch amplification mutation PCR assay was developed and validated for rapid detection of the biotype specific cholera toxin B subunit of V. cholerae O1. This assay will enable easy monitoring of the spread of a new emerging variant of the El Tor biotype of V. cholerae O1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Bacterial Typing Techniques / methods*
  • Cholera Toxin / genetics*
  • DNA Primers
  • Feasibility Studies
  • Genes, Bacterial / genetics
  • Polymerase Chain Reaction / methods*
  • Vibrio cholerae O1 / classification*
  • Vibrio cholerae O1 / genetics*


  • DNA Primers
  • Cholera Toxin