Effects of hyperbaric oxygen therapy on tumor growth in murine model of PC-3 prostate cancer cell line

Urology. 2009 Jan;73(1):205-8. doi: 10.1016/j.urology.2008.04.057. Epub 2008 Jun 25.


Objectives: To test the hypothesis that hyperbaric oxygen (HBO) has no effect on tumor growth in a murine model of indolent in vivo prostate cancer. HBO means breathing pure (100%) oxygen under increased atmospheric pressure.

Methods: Human prostate PC-3 cells were injected into 40 severe combined-immunodeficient mice. They were randomized to undergo 20 sessions of either HBO or normobaric air in standardized conditions and observed for 4 weeks before histologic assessment of any palpable tumors that had developed. The analysis of the developed PC-3 tumors included tumor volume, microvessel density, apoptosis-associated markers (ie, p53, p27), and the proliferative index (Ki-67).

Results: The exposure to HBO at 2 atm for 20 treatment sessions, which comprised a daily 90-minute session, 5 d/wk, had no effect on the prostate cancer volume (P > .05). No differences were observed in tumor microvessel density, proliferative index, or apoptosis markers compared with the non-HBO group (P > .05).

Conclusions: HBO did not have a tumor stimulatory effect on prostate cancer and could potentially be used safely in conjunction with other therapeutic modalities.

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Disease Models, Animal
  • Hyperbaric Oxygenation*
  • Male
  • Mice
  • Prostatic Neoplasms / pathology*