OPCML gene promoter methylation and gene expression in tumor and stroma cells of invasive cervical carcinoma

Cancer Invest. 2008 Jul;26(6):569-74. doi: 10.1080/07357900701837044.


To investigate the CpG island methylation and the mRNA expression of OPCML gene in patients with cervical carcinoma, we collected tumor and stroma cells from 36 invasive cervical carcinoma samples and 16 normal cervical tissues as well as Hela cells. Methylation specific PCR was used to detect promoter CpG island methylation status, and fluorescence quantitative RT-PCR was used to detection of OPCML gene expression. Our data showed that OPCML gene promoter methylation may play an important role in the carcinogenesis of cervical carcinoma and OPCML gene may be a cervical carcinoma-associated candidate TSG (tumor suppressor gene).

MeSH terms

  • Adult
  • Carcinoma / genetics*
  • Carcinoma / metabolism
  • Carcinoma / pathology
  • Cell Adhesion Molecules / genetics*
  • Cell Adhesion Molecules / metabolism
  • CpG Islands*
  • DNA Methylation*
  • Female
  • GPI-Linked Proteins
  • Gene Expression Regulation, Neoplastic*
  • Genes, Tumor Suppressor*
  • HeLa Cells
  • Humans
  • Middle Aged
  • Neoplasm Invasiveness
  • Polymerase Chain Reaction
  • Promoter Regions, Genetic*
  • RNA, Messenger / metabolism
  • Stromal Cells / metabolism
  • Stromal Cells / pathology
  • Uterine Cervical Neoplasms / genetics*
  • Uterine Cervical Neoplasms / metabolism
  • Uterine Cervical Neoplasms / pathology


  • Cell Adhesion Molecules
  • GPI-Linked Proteins
  • OPCML protein, human
  • RNA, Messenger