Abstract
We studied the effects of isoflavones and irradiation on cell cycle in a human salivary gland cell line (HSG). Genistein and a soy isoflavone conjugate (NS) inhibited DNA synthesis. Cells deconjugated the glucoside form of isoflavones in NS to the aglycones genistein and daidzein. NS, genistein and IR increased phosphorylation of p53 and p21 CIP1 at serine 15 (phos-p53). Irradiation and NS also increased levels of p21 CIP1. In a cologenic survival assay, cells in log phase growth had high radio-sensitivity with 2 Gy causing a reduction in survival (SF2=0.45).
Conclusion:
isoflavones and radiation may interact to sensitize cancer cells to radiation.
MeSH terms
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Cell Cycle / drug effects
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Cell Line
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Cell Proliferation / drug effects
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Cell Survival / drug effects
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Cell Survival / radiation effects
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Cyclin-Dependent Kinase Inhibitor p21 / analysis
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Cyclin-Dependent Kinase Inhibitor p27
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DNA Damage
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Dose-Response Relationship, Drug
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Gamma Rays*
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Genistein / pharmacology*
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Glycine max / chemistry*
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Humans
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Intracellular Signaling Peptides and Proteins / analysis
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Isoflavones / pharmacology*
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Phosphorylation
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Salivary Glands / cytology
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Salivary Glands / drug effects*
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Salivary Glands / radiation effects
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Tumor Suppressor Protein p53 / analysis
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Vitamin E / pharmacology
Substances
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CDKN1A protein, human
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CDKN1B protein, human
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Cyclin-Dependent Kinase Inhibitor p21
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Intracellular Signaling Peptides and Proteins
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Isoflavones
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Tumor Suppressor Protein p53
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Vitamin E
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Cyclin-Dependent Kinase Inhibitor p27
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Genistein