Streptococcus pyogenes degrades extracellular matrix in chondrocytes via MMP-13

Biochem Biophys Res Commun. 2008 Aug 29;373(3):450-4. doi: 10.1016/j.bbrc.2008.06.061. Epub 2008 Jun 27.


Group A streptococcus (GAS) causes a wide range of human diseases, including bacterial arthritis. The pathogenesis of arthritis is characterized by synovial proliferation and the destruction of cartilage and subchondral bone in joints. We report here that GAS strain JRS4 invaded a chondrogenic cell line ATDC5 and induced the degradation of the extracellular matrix (ECM), whereas an isogenic mutant of JRS4 lacking a fibronectin-binding protein, SAM1, failed to invade the chondrocytes or degrade the ECM. Reverse transcription-PCR and Western blot analysis revealed that the expression of matrix metalloproteinase (MMP)-13 was strongly elevated during the infection with GAS. A reporter assay revealed that the activation of the AP-1 transcription factor and the phosphorylation of c-Jun terminal kinase participated in MMP-13 expression. These results suggest that MMP-13 plays an important role in the destruction of infected joints during the development of septic arthritis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arthritis, Infectious / enzymology*
  • Cell Line
  • Chondrocytes / enzymology*
  • Chondrocytes / microbiology
  • Chondrocytes / ultrastructure
  • Extracellular Matrix / metabolism*
  • Extracellular Matrix / microbiology
  • JNK Mitogen-Activated Protein Kinases / metabolism
  • Matrix Metalloproteinase 13 / metabolism*
  • Mice
  • Streptococcal Infections / enzymology*
  • Streptococcus pyogenes*
  • Transcription Factor AP-1 / metabolism


  • Transcription Factor AP-1
  • JNK Mitogen-Activated Protein Kinases
  • Matrix Metalloproteinase 13