Antibody detection and cross-reactivity among species and serogroups of Legionella by indirect immunofluorescence test

S Ditommaso; M Giacomuzzi; M Gentile; C M Zotti

doi:10.1016/j.mimet.2008.06.002

Antibody detection and cross-reactivity among species and serogroups of Legionella by indirect immunofluorescence test

J Microbiol Methods. 2008 Oct;75(2):350-3. doi: 10.1016/j.mimet.2008.06.002. Epub 2008 Jun 11.

Authors

S Ditommaso¹, M Giacomuzzi, M Gentile, C M Zotti

Affiliation

¹ Dipartimento di Sanità Pubblica e di Microbiologia, Università degli Studi di Torino, Via Santena 5 bis 10126 Torino, Italy. savina.ditommaso@unito.it

PMID: 18586056
DOI: 10.1016/j.mimet.2008.06.002

Abstract

The purpose of the present study was to determine possible cross-reactivity between different serogroups or between different species by means of bacterial smears of individual L. pneumophila serogroups and several non-pneumophila Legionella spp. Using BIOCHIP slide we found a considerable frequency of cross-reactions with L. pneumophila serogroup 12 (88.4%) therefore BIOCHIP slide proved as useful method for identification of cross-reactivity between members of the Legionnellaceae.

MeSH terms

Antibodies, Bacterial / blood*
Antibodies, Bacterial / immunology
Antigens, Bacterial / immunology*
Cross Reactions
Fluorescent Antibody Technique, Indirect / methods*
Humans
Legionella / classification*
Legionella / immunology*
Legionella pneumophila / classification
Legionella pneumophila / immunology*
Legionellosis / diagnosis
Legionellosis / immunology
Legionnaires' Disease / diagnosis
Legionnaires' Disease / immunology
Protein Array Analysis / methods
Serotyping

Substances

Antibodies, Bacterial
Antigens, Bacterial