14-3-3 regulates the G2/M transition in the basidiomycete Ustilago maydis

Fungal Genet Biol. 2008 Aug;45(8):1206-15. doi: 10.1016/j.fgb.2008.05.010. Epub 2008 Jun 3.

Abstract

14-3-3 proteins are a family of highly conserved polypeptides that function as small adaptors that facilitate a diverse array of cellular processes by binding phosphorylated target proteins. One of these processes is the regulation of the cell cycle. Here we characterized the role of Bmh1, a 14-3-3 protein, in the cell cycle regulation of the fungus Ustilago maydis. We found that this protein is essential in U. maydis and that it has roles during the G2/M transition in this organism. The function of 14-3-3 in U. maydis seems to mirror the proposed role for this protein during Schizosaccharomyces pombe cell cycle regulation. We provided evidence that in U. maydis 14-3-3 protein binds to the mitotic regulator Cdc25. Comparison of the roles of 14-3-3 during cell cycle regulation in other fungal system let us to discuss the connections between morphogenesis, cell cycle regulation and the evolutionary role of 14-3-3 proteins in fungi.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 14-3-3 Proteins / genetics
  • 14-3-3 Proteins / metabolism
  • 14-3-3 Proteins / physiology*
  • Amino Acid Sequence
  • Blotting, Northern
  • Blotting, Western
  • Cell Cycle / genetics
  • Cell Cycle / physiology*
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Fungal Proteins / physiology*
  • G2 Phase / genetics
  • G2 Phase / physiology
  • Gene Expression Regulation, Fungal
  • Immunoprecipitation
  • Mitosis / genetics
  • Mitosis / physiology
  • Molecular Sequence Data
  • Mutation
  • Protein Binding
  • Sequence Homology, Amino Acid
  • Ustilago / cytology
  • Ustilago / genetics
  • Ustilago / physiology*
  • cdc25 Phosphatases / genetics
  • cdc25 Phosphatases / metabolism

Substances

  • 14-3-3 Proteins
  • Fungal Proteins
  • cdc25 Phosphatases