Antibody Suspension Bead Arrays Within Serum Proteomics

J Proteome Res. 2008 Aug;7(8):3168-79. doi: 10.1021/pr700890b. Epub 2008 Jun 28.

Abstract

Antibody microarrays offer a powerful tool to screen for target proteins in complex samples. Here, we describe an approach for systematic analysis of serum, based on antibodies and using color-coded beads for the creation of antibody arrays in suspension. This method, adapted from planar antibody arrays, offers a fast, flexible, and multiplexed procedure to screen larger numbers of serum samples, and no purification steps are required to remove excess labeling substance. The assay system detected proteins down to lower picomolar levels with dynamic ranges over 3 orders of magnitude. The feasibility of this workflow was shown in a study with more than 200 clinical serum samples tested for 20 serum proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies*
  • Antibody Specificity
  • Antigens / analysis*
  • Antigens / immunology
  • Blood Proteins / analysis*
  • Blood Proteins / immunology
  • Feasibility Studies
  • Humans
  • Protein Array Analysis
  • Proteome / analysis*
  • Proteome / immunology
  • Sensitivity and Specificity
  • Serum

Substances

  • Antibodies
  • Antigens
  • Blood Proteins
  • Proteome