Molecular and immunological characterization of novel weed pollen pan-allergens

Allergy. 2008 Jul;63(7):872-81. doi: 10.1111/j.1398-9995.2008.01635.x.


Background: Pan-allergens like profilins, calcium-binding proteins (CBPs), and nonspecific lipid transfer proteins have been suggested as possible specific markers for multiple pollen sensitizations, and could be used to predict cross-sensitization/poly-sensitization to several pollen allergens. Therefore, the purification and characterization of cross-reacting allergens in pollen is an extremely important task towards correct allergy diagnosis.

Methods: New pan-allergens were identified by screening a ragweed pollen cDNA library with sera of patients allergic to mugwort pollen. Resulting proteins were cloned, expressed, purified and characterized.

Results: We report complete cDNA sequences of two profilin isoforms (Amb a 8.01 and Amb a 8.02), two isoforms of a 2EF-hand CBP (Amb a 9.01 and Amb a 9.02), a new 3EF-hand CBP (Amb a 10) from ragweed pollen and a 2EF-hand CBP from mugwort (Art v 5). All these proteins were expressed in Escherichia coli, purified to homogeneity and characterized by biochemical and immunological means.

Conclusions: The identified proteins are novel pan-allergens and can be used as diagnostic markers for polysensitization and used in component-resolved diagnosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allergens / genetics
  • Allergens / immunology*
  • Ambrosia / genetics
  • Ambrosia / immunology*
  • Amino Acid Sequence
  • Animals
  • Antigens, Plant / genetics
  • Antigens, Plant / immunology
  • Artemisia / genetics
  • Artemisia / immunology
  • Basophil Degranulation Test
  • Basophils / immunology
  • Calcium-Binding Proteins / genetics
  • Calcium-Binding Proteins / immunology*
  • Cell Line
  • Circular Dichroism
  • Cross Reactions
  • EF Hand Motifs
  • Electrophoresis, Polyacrylamide Gel
  • Gene Library
  • Humans
  • Immunoglobulin E / immunology
  • Molecular Sequence Data
  • Pollen / genetics
  • Pollen / immunology*
  • Profilins / genetics
  • Profilins / immunology*
  • RNA, Plant / analysis
  • Rats
  • Rhinitis, Allergic, Seasonal / blood


  • Allergens
  • Antigens, Plant
  • Calcium-Binding Proteins
  • Profilins
  • RNA, Plant
  • Immunoglobulin E