Objective: To study the effects of Hemp seeds protein (HSP) on antifatigue and immunnomodulation effect in mice.
Methods: Female ICR mice at the ages of 6-8 weeks were administered with the HSP for 4 weeks at the dose of 1.32, 2.64 and 7.92 g/kg bw. After four weeks' treatment, the mice swimming time and some hiochemistry indexes were tested, such as blood lactic acid, BUN and hepatic glycogen, as well as the ratio of spleen and thymus organs index, the capacities of lymphocyte proliferation induced by ConA, DTH response, IgM-PFC number, the level of serum HC50, the indexes of the chicken red blood cells phagocytosis and the clearance rate of carbon particles were detected, and the ratio of CD4+ T helper (Th) cell in spleen were determined by the flow cytometer.
Results: In comparison with the control group, the HSP could improve the the swimming time and contents of liver hepatin distinctly, and reduced the contents of blood lactic acid. Then the capacities of lymphocyte proliferation induced by ConA, DTH response, IgM-PFC number, the level of serum HC50, the indexes of the clearance rate of carbon particles and the ratio of CD4+ T helper (Th) cell in spleen increased.
Conclusion: HSP could have the ability of antifatigue and improve the immunomodulation effect in mice.