The naturally occurring flavolignan, deoxypodophyllotoxin, inhibits lipopolysaccharide-induced iNOS expression through the NF-kappaB activation in RAW264.7 macrophage cells

Biol Pharm Bull. 2008 Jul;31(7):1312-5. doi: 10.1248/bpb.31.1312.


Deoxypodophyllotoxin (DPT), a naturally occurring flavolignan with anti-inflammatory activity, was isolated from Anthriscus sylvestris HOFFM., and we examined its effects on the expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-stimulated, murine macrophage-like RAW264.7 cells. Western blot analysis performed with specific anti-iNOS antibodies showed that a decrease in nitric oxide (NO) was accompanied by a decrease in the iNOS protein level. To clarify the mechanistic basis for DPT's ability to inhibit iNOS induction, we examined the effect of DPT on nuclear factor (NF)-kappaB transcriptional activity and DNA binding activity. DPT potently suppressed both reporter gene activity and DNA binding activity. These findings suggest that DPT in RAW264.7 cells abolished LPS-induced iNOS expression by inhibiting the transcription factor, NF-kappaB.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apiaceae / chemistry
  • Blotting, Western
  • Cell Line
  • Chromatography, High Pressure Liquid
  • Chromatography, Thin Layer
  • DNA / metabolism
  • Drugs, Chinese Herbal
  • Electrophoresis, Polyacrylamide Gel
  • Electrophoretic Mobility Shift Assay
  • Indicators and Reagents
  • Lipopolysaccharides / antagonists & inhibitors*
  • Lipopolysaccharides / toxicity
  • Luciferases / genetics
  • Macrophages / drug effects
  • Macrophages / enzymology
  • Macrophages / metabolism*
  • Magnetic Resonance Spectroscopy
  • Mice
  • Nitric Oxide Synthase Type II / biosynthesis*
  • Plant Roots / chemistry
  • Podophyllotoxin / analogs & derivatives*
  • Podophyllotoxin / pharmacology
  • Transcription Factor RelA / metabolism*
  • Transfection


  • Drugs, Chinese Herbal
  • Indicators and Reagents
  • Lipopolysaccharides
  • Transcription Factor RelA
  • deoxypodophyllotoxin
  • DNA
  • Luciferases
  • Nitric Oxide Synthase Type II
  • Podophyllotoxin