Sequential and quantitative analysis of a murine model of elastase-induced emphysema

Biol Pharm Bull. 2008 Jul;31(7):1434-8. doi: 10.1248/bpb.31.1434.


Emphysema, one of chronic obstructive pulmonary disease (COPD), is characterized as destruction of airway wall and small airway inflammation. To assess the kinetics of disease progression in murine model of elastase-induced emphysema, we used micro-computed tomography (CT) compared with morphological changes. Two week after elastase administration, a significant increase in the volume of low-density areas, recognized as -800--600 Hounsfield units by micro-CT, was observed. Coefficient of correlation between mean linear intercept (Lm) and low-density area examined by CT, was 0.79 (p<0.01). Micro-CT can quantitatively and sequentially detect murine emphysematous changes, offering a practical method to sequentially analyze the therapeutic effects of treatments in a murine model of emphysema.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bronchoalveolar Lavage Fluid / cytology
  • Emphysema / chemically induced*
  • Emphysema / pathology*
  • Image Processing, Computer-Assisted
  • Kinetics
  • Mice
  • Mice, Inbred C57BL
  • Pancreatic Elastase*
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Tomography, X-Ray Computed


  • RNA, Messenger
  • Pancreatic Elastase