Sequential and quantitative analysis of a murine model of elastase-induced emphysema

Biol Pharm Bull. 2008 Jul;31(7):1434-8. doi: 10.1248/bpb.31.1434.

Abstract

Emphysema, one of chronic obstructive pulmonary disease (COPD), is characterized as destruction of airway wall and small airway inflammation. To assess the kinetics of disease progression in murine model of elastase-induced emphysema, we used micro-computed tomography (CT) compared with morphological changes. Two week after elastase administration, a significant increase in the volume of low-density areas, recognized as -800--600 Hounsfield units by micro-CT, was observed. Coefficient of correlation between mean linear intercept (Lm) and low-density area examined by CT, was 0.79 (p<0.01). Micro-CT can quantitatively and sequentially detect murine emphysematous changes, offering a practical method to sequentially analyze the therapeutic effects of treatments in a murine model of emphysema.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bronchoalveolar Lavage Fluid / cytology
  • Emphysema / chemically induced*
  • Emphysema / pathology*
  • Image Processing, Computer-Assisted
  • Kinetics
  • Mice
  • Mice, Inbred C57BL
  • Pancreatic Elastase*
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Tomography, X-Ray Computed

Substances

  • RNA, Messenger
  • Pancreatic Elastase