The impact of high anxiety level on the oxidative status of mouse peripheral blood lymphocytes, granulocytes and monocytes

Eur J Pharmacol. 2008 Jul 28;589(1-3):173-5. doi: 10.1016/j.ejphar.2008.06.053. Epub 2008 Jun 18.


Anxiety has been implicated in a number of psychiatric disorders, such as generalized anxiety disorder, depression, panic attacks, phobias, obsessive-compulsive disorders and posttraumatic stress disorders. In this study, we investigated the impact of high anxiety on the levels of intracellular reactive oxygen species in lymphocytes, granulocytes and monocytes from the peripheral blood of mice by using a 2',7'-dichlorofluorescein diacetate (DCFH-DA) probe. The behavioural light/dark choice test was used to distinguish highly anxious from less anxious mice. Our results showed that a high anxiety level significantly increased the generation of reactive oxygen species in the peripheral blood lymphocytes, granulocytes and monocytes.

MeSH terms

  • Animals
  • Anxiety / metabolism*
  • Anxiety / psychology
  • Behavior, Animal
  • Choice Behavior
  • Disease Models, Animal
  • Flow Cytometry
  • Fluoresceins
  • Fluorescent Dyes
  • Granulocytes / metabolism*
  • Lymphocytes / metabolism*
  • Male
  • Mice
  • Monocytes / metabolism*
  • Oxidation-Reduction
  • Oxidative Stress*
  • Reactive Oxygen Species / metabolism*


  • Fluoresceins
  • Fluorescent Dyes
  • Reactive Oxygen Species
  • diacetyldichlorofluorescein