Virion disruption by ozone-mediated reactive oxygen species

J Virol Methods. 2008 Oct;153(1):74-7. doi: 10.1016/j.jviromet.2008.06.004. Epub 2008 Jul 24.


It is well documented in the scientific literature that ozone-oxygen mixtures inactivate microorganisms including bacteria, fungi and viruses (Hoff, J.C., 1986. Inactivation of microbial agents by chemical disinfectants. EPA 600 S2-86 067. Office of Water, U.S. Environmental Protection Agency, Washington, DC; Khadre, M.A., Yousef, A.E., Kim, J.-G., 2001. Microbiological aspects of ozone applications in food: a review. J. Food Sci. 66, 1242-1252). In the current study, delivery and absorption of precisely known concentrations of ozone (in liquid media) were used to inactivate virus infectivity. An ozone-oxygen delivery system capable of monitoring and recording ozone concentrations in real time was used to inactivate a series of enveloped and non-enveloped viruses including herpes simplex virus type-1 (HHV-1, strain McIntyre), vesicular stomatitis Indiana virus (VSIV), vaccinia virus (VACV, strain Elstree), adenovirus type-2 (HAdV-2), and the PR8 strain of influenza A virus (FLUAVA/PR/8/34/H1N1; FLUAV). The results of the study showed that ozone exposure reduced viral infectivity by lipid peroxidation and subsequent lipid envelope and protein shell damage. These data suggest that a wide range of virus types can be inactivated in an environment of known ozone exposure.

MeSH terms

  • Adenoviridae / drug effects
  • Adenoviridae / ultrastructure
  • Disinfectants / pharmacology*
  • Influenza A virus / drug effects
  • Influenza A virus / ultrastructure
  • Microscopy, Electron, Transmission
  • Ozone / pharmacology*
  • Reactive Oxygen Species / pharmacology*
  • Simplexvirus / drug effects
  • Simplexvirus / ultrastructure
  • Vaccinia virus / drug effects
  • Vaccinia virus / ultrastructure
  • Vesicular stomatitis Indiana virus / drug effects
  • Vesicular stomatitis Indiana virus / ultrastructure
  • Viral Plaque Assay
  • Virion / drug effects*
  • Virion / ultrastructure
  • Virus Inactivation*


  • Disinfectants
  • Reactive Oxygen Species
  • Ozone