Correlation of MMP(1) and TIMP (1) Expression With Pituitary Adenoma Fibrosis

J Neurooncol. 2008 Nov;90(2):151-6. doi: 10.1007/s11060-008-9647-9. Epub 2008 Jul 5.

Abstract

Objective: To explore the correlation of matrix metalloproteinase-1 (MMP(1)) and tissue inhibitor of metalloproteinase-1 (TIMP(1)) with pituitary adenoma fibrosis.

Methods: Thirty-eight pituitary adenoma specimens were divided into fibrous group (6 patients) and non-fibrous group (32 patients). MMP(1) and TIMP(1) expression was detected by RT-PCR and immunohistochemistry. The collagen content was determined by Sirius scarlet staining.

Results: In fibrous and non-fibrous group: (1) the collagen content was 20.95 +/- 8.42% and 7.98 +/- 5.18% respectively, and there was a statistical significance (P < 0.01). (2) The expression of MMP(1)mRNA was 0.47 +/- 0.40 and 0.59 +/- 0.54 respectively and its protein expression was 0.12 +/- 0.09 and 0.13 +/- 0.09 respectively, and there was no statistical significance (P > 0.05). Their expression was not related to collagen content (P > 0.05). (3) The expression of TIMP(1)mRNA was 1.61 +/- 1.09 and 0.79 +/- 0.59 respectively and its protein expression was 0.58 +/- 0.11 and 0.32 +/- 0.18 respectively, and there was a statistical significance (P < 0.01). Their expression was related to collagen content (P < 0.01).

Conclusion: The pathological features of pituitary adenoma fibrosis are excessive collagen deposition. High expression of TIMP(1) may be an important cause.

MeSH terms

  • Adenoma / metabolism*
  • Adenoma / pathology
  • Adult
  • Aged
  • Collagen / metabolism
  • Female
  • Fibrosis
  • Gene Expression
  • Humans
  • Male
  • Matrix Metalloproteinase 1 / genetics
  • Matrix Metalloproteinase 1 / metabolism*
  • Middle Aged
  • Pituitary Neoplasms / metabolism*
  • Pituitary Neoplasms / pathology
  • RNA, Messenger / metabolism
  • Retrospective Studies
  • Tissue Inhibitor of Metalloproteinase-1 / genetics
  • Tissue Inhibitor of Metalloproteinase-1 / metabolism*
  • Young Adult

Substances

  • RNA, Messenger
  • Tissue Inhibitor of Metalloproteinase-1
  • Collagen
  • Matrix Metalloproteinase 1