VEGF expression and the effect of NSAIDs on ascites cell proliferation in the hen model of ovarian cancer

Gynecol Oncol. 2008 Sep;110(3):418-24. doi: 10.1016/j.ygyno.2008.05.018. Epub 2008 Jul 7.

Abstract

Objectives: We aimed to determine the expression of vascular endothelial growth factor (VEGF) and the effect of nonsteroidal anti-inflammatory drugs (NSAIDs) on the proliferation of cells isolated from ascites in the hen model of ovarian cancer.

Methods: Ovarian tumor and normal ovary were collected from hens and ascites cells were isolated from hens with ovarian cancer. Quantitative real-time PCR was used to quantify mRNA expression. Immunohistochemical and/or Western blot analyses were used to localize protein expression in ovarian tumors, normal ovaries, and ascites cells. Cells were treated with a nonspecific, COX-1-specific, or COX-2-specific NSAID and proliferation was determined.

Results: VEGF mRNA was increased in ascites cells and there was a trend for a correlation between VEGF mRNA in ascites cells and ascites volume. VEGF protein was localized to theca cells of normal ovaries, in glandular areas of tumors, and to the cytoplasm of ascites cells. Aspirin and a COX-1-specific inhibitor decreased the proliferation of ascites cells, whereas a COX-2-specific inhibitor did not.

Conclusions: VEGF may play a role in ovarian cancer progression in the hen and the proliferation of ascites cells can be decreased by targeting the COX-1 but not COX-2 pathway.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
  • Ascites / drug therapy
  • Ascites / pathology
  • Aspirin / pharmacology
  • Cell Growth Processes / drug effects
  • Chickens
  • Cyclooxygenase 1 / biosynthesis
  • Cyclooxygenase 1 / genetics
  • Cyclooxygenase 2 / biosynthesis
  • Cyclooxygenase 2 / genetics
  • Cyclooxygenase Inhibitors / pharmacology
  • Dinoprostone / metabolism
  • Disease Models, Animal
  • Female
  • Nitrobenzenes / pharmacology
  • Ovarian Neoplasms / drug therapy*
  • Ovarian Neoplasms / genetics
  • Ovarian Neoplasms / metabolism
  • Ovarian Neoplasms / pathology
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Sulfonamides / pharmacology
  • Tumor Cells, Cultured
  • Vascular Endothelial Growth Factor A / biosynthesis*
  • Vascular Endothelial Growth Factor A / genetics

Substances

  • Anti-Inflammatory Agents, Non-Steroidal
  • Cyclooxygenase Inhibitors
  • Nitrobenzenes
  • RNA, Messenger
  • Sulfonamides
  • Vascular Endothelial Growth Factor A
  • N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide
  • Cyclooxygenase 1
  • Cyclooxygenase 2
  • Dinoprostone
  • Aspirin