Effect of human papilloma virus expression on clinical course of laryngeal papilloma

Acta Otolaryngol. 2008 Oct;128(10):1138-44. doi: 10.1080/00016480701827509.


Conclusion: Our observations suggest that human papilloma virus (HPV) 6/11 is the main causative agent of laryngeal papilloma and that detection of active HPV DNA expression may be helpful in identifying patients with aggressive recurrent laryngeal papilloma.

Objectives: HPV is assumed to be the main causative agent of this disease. We investigated the expression of the entire genotype of HPV in cases of laryngeal papilloma and correlated their expression with the clinical course of the disease.

Subjects and methods: Seventy cases of laryngeal papilloma were evaluated for the presence of the HPV genome by in situ hybridization (ISH) using wide-spectrum HPV DNA probe. Specific types of HPV infection were determined by DNA ISH using type-specific HPV DNA probes (HPV 6, 11, 16, 18, 31, 33). Separate analyses were conducted comparing viral types, frequency of recurrences and duration of disease-free periods.

Results: We detected HPV DNA in 40 of the 70 laryngeal papilloma cases (57%). In particular, HPV DNA was detected in 75% of the juvenile types. There were significant associations between HPV and laryngeal papilloma (p<0.01). Among the HPV-positive cases, major specific types were HPV 6/11 (97%). Significant associations were also noted between viral expression and clinical course.

MeSH terms

  • Adult
  • Child
  • DNA Probes
  • DNA, Viral / genetics
  • Disease-Free Survival
  • Female
  • Humans
  • In Situ Hybridization
  • Laryngeal Neoplasms / mortality
  • Laryngeal Neoplasms / pathology
  • Laryngeal Neoplasms / virology*
  • Male
  • Neoplasm Recurrence, Local / virology
  • Papilloma / mortality
  • Papilloma / pathology
  • Papilloma / virology*
  • Papillomaviridae / isolation & purification
  • Papillomavirus Infections / virology*
  • Remission Induction
  • Retrospective Studies


  • DNA Probes
  • DNA, Viral