Inhibition of CD147 gene expression via RNA interference reduces tumor cell proliferation, activation, adhesion, and migration activity in the human Jurkat T-lymphoma cell line

Cancer Invest. 2008 Aug;26(7):689-97. doi: 10.1080/07357900701867892.


CD147, a leukocyte surface molecule over-expressed in T-lymphoma cells, is reportedly associated with lymphocyte activation and proteinase production via interactions with fibroblasts and plays a role in stromal invasion by lymphoma cells. To determine the role of CD147 in the progression of T-lymphoma, we performed siRNA interference-mediated knockdown of CD147 in a CD147-expressing Jurkat T-cell line. CD147 knockdown resulted in the decreased proliferation and migration of Jurkat cells and reduced the adhesion of Jurkat cells to extracelluar matrix fibronectin in vitro. CD147-siRNA inhibited the activation of Jurkat cells via down-regulation of CD25 expression. Our results indicate that CD147 is involved in T-lymphoma progression, a finding useful in efforts to develop targeted therapies to treat patients with T-lymphoma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Basigin / genetics
  • Basigin / metabolism*
  • Cell Adhesion* / genetics
  • Cell Adhesion* / immunology
  • Cell Movement* / genetics
  • Cell Movement* / immunology
  • Cell Proliferation*
  • Down-Regulation
  • Fibronectins / metabolism
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Interleukin-2 Receptor alpha Subunit / metabolism
  • Jurkat Cells
  • Lymphocyte Activation* / genetics
  • Lymphoma, T-Cell / genetics
  • Lymphoma, T-Cell / immunology*
  • Lymphoma, T-Cell / metabolism
  • Lymphoma, T-Cell / pathology
  • Middle Aged
  • RNA Interference*
  • RNA, Small Interfering / metabolism
  • Transfection


  • BSG protein, human
  • Fibronectins
  • Interleukin-2 Receptor alpha Subunit
  • RNA, Small Interfering
  • Basigin