Identification and initial characterization of the eutF locus of Salmonella typhimurium

J Bacteriol. 1991 Aug;173(16):5168-72. doi: 10.1128/jb.173.16.5168-5172.1991.


We report the isolation and initial characterization of mutations in the newly described eutF locus of Salmonella typhimurium LT2. Mutations in eutF render a strain unable to utilize ethanolamine as a source of carbon and/or energy and impair growth on ethanolamine as a sole nitrogen source. Strains carrying eutF mutations exhibit a 2-order-of-magnitude decrease in transcription of the unlinked eutDEABCR operon (50 min), which codes for the enzymes needed to catabolize ethanolamine; have only 10% of the ethanolamine ammonia-lyase activity found in the wild type; and show a marked reduction in the rate of ethanolamine uptake. Deletion mapping and three-factor cross analysis results are consistent with the gene order cobA trp eutF tonB at 34 min on the linkage map. We discuss two possible roles for the EutF protein: (i) as an ethanolamine permease or (ii) as a transcription factor required for the expression of the eutDEABCR operon.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromosome Mapping
  • Crosses, Genetic
  • Ethanolamine
  • Ethanolamine Ammonia-Lyase / metabolism*
  • Ethanolamines / metabolism*
  • Kinetics
  • Membrane Transport Proteins / metabolism
  • Mutation / genetics
  • Operon / genetics
  • Phenotype
  • Salmonella typhimurium / genetics*
  • Salmonella typhimurium / isolation & purification
  • Salmonella typhimurium / metabolism
  • Transcription Factors / metabolism


  • Ethanolamines
  • Membrane Transport Proteins
  • Transcription Factors
  • Ethanolamine
  • Ethanolamine Ammonia-Lyase