Comparative inhibition studies of enoyl-CoA hydratase 1 and enoyl-CoA hydratase 2 in long-chain fatty acid oxidation

Org Lett. 2008 Aug 7;10(15):3355-8. doi: 10.1021/ol801267e. Epub 2008 Jul 9.

Abstract

Enoyl-CoA hydratase 1 and enoyl-CoA hydratase 2 in long-chain fatty acid oxidation were comparatively investigated through mechanistic studies for inactivation of the enzymes with methylenecyclopropylformyl-CoA and 3-octynoyl-CoA. Methylenecyclopropylformyl-CoA can inactivate both enzymes, while 3-octynoyl-CoA inactivates enoyl-CoA hydratase 2 only. The study increased our understanding of these two enzymes in fatty acid oxidation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acyl Coenzyme A / chemistry*
  • Acyl Coenzyme A / pharmacology
  • Amino Acid Sequence
  • Animals
  • Enoyl-CoA Hydratase / antagonists & inhibitors*
  • Enoyl-CoA Hydratase / chemistry
  • Enoyl-CoA Hydratase / metabolism*
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / pharmacology
  • Fatty Acids / chemistry
  • Fatty Acids / metabolism*
  • Isoenzymes / antagonists & inhibitors
  • Isoenzymes / chemistry
  • Isoenzymes / metabolism
  • Mitochondria / enzymology
  • Molecular Sequence Data
  • Oxidation-Reduction
  • Peroxisomes / chemistry
  • Peroxisomes / metabolism
  • Rats
  • Tandem Mass Spectrometry

Substances

  • (methylenecyclopropyl)formyl coenzyme A
  • 3-octynoyl-CoA
  • Acyl Coenzyme A
  • Enzyme Inhibitors
  • Fatty Acids
  • Isoenzymes
  • Enoyl-CoA Hydratase