The effects of hyperbaric oxygen therapy on colonic anastomosis in rats with peritonitis

J Invest Surg. 2008 Jul-Aug;21(4):195-200. doi: 10.1080/08941930802155534.


The aim of this study is to investigate the healing effect of hyperbaric oxygen (HBO) on colonic anastomoses in the presence of experimentally induced peritonitis. Thirty-two rats were allocated randomly into short-term anastomosis (STA), short-term anastomosis + HBO treatment (STA+HBO), long-term anastomosis (LTA), and long-term anastomosis + HBO (LTA+HBO) treatment groups. The STA and LTA groups were administered fluid resuscitation and antibiotics for 3 and 7 days, respectively, whereas the HBO treatment groups received additional HBO therapy for 3 and 7 days, respectively. The rats were reoperated on the third and the seventh days of anostomoses for evaluation. The bursting pressures in STA+HBO and LTA+HBO therapy groups were significantly higher than those in groups with anastomoses alone (p < .001 and p < .01). HBO therapy did not affect the fibrotic index neither in STA nor in LTA groups (p > .05 for both); however, it was significantly higher in LTA+HBO group than that in STA+HBO group (p < .05). The hydroxyproline level was significantly higher in LTA group than in STA group (p < .05), yet HBO therapy did not affect the hydroxyproline levels in STA or LTA groups (p > .05 for both). It is concluded that hyperbaric oxygen treatment has positive effects on colonic anastomotic healing in case of peritonitis.

MeSH terms

  • Anastomosis, Surgical*
  • Animals
  • Colon / metabolism
  • Colon / pathology
  • Colon / surgery*
  • Female
  • Fibrosis / pathology
  • Hydroxyproline / metabolism
  • Hyperbaric Oxygenation*
  • Male
  • Peritonitis / pathology*
  • Postoperative Complications / prevention & control
  • Pressure
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Tensile Strength
  • Time Factors
  • Wound Healing


  • Hydroxyproline