Characteristics of collagenase-2 from gingival crevicular fluid and peri-implant sulcular fluid in periodontitis and peri-implantitis patients: pilot study

Acta Odontol Scand. 2008 Aug;66(4):219-24. doi: 10.1080/00016350802183393.


Objective: To compare collagenase activity and collagenolytic matrix metalloproteinase (MMP) levels in gingival crevicular fluid (GCF) and in peri-implant sulcular fluid (PISF) in gingivitis (G), chronic periodontitis (CP), and peri-implantitis (PI) human subjects.

Material and methods: GCF and PISF were collected on filter paper strips, volume was determined, and samples were extracted in buffer containing general proteinase but not MMP inhibitors. Collagenase activity was measured using a DNP-synthetic octapeptide, and molecular and activation forms of collagenase-2 by Western immunoblotting.

Results: GCF from CP and G sites exhibited elevated collagenase activity and flow, but collagenase concentrations expressed per microl were not significantly different between the healthy and G sites. Minimal fluid was obtained from healthy PISF, and collagenase concentration was the same or lower than in healthy GCF. Although PISF flow was 34% lower than GCF flow in CP subjects, collagenase concentration in CP and in PI sites was 78% and 971% greater, respectively, than in the appropriate healthy sites. Western immunoblot revealed MMP-8 in both PISF and GCF; fibroblast-type MMP-8 was not detected in healthy GCF and PISF. Immunoreactivity level and inactive and activated forms of PMN-type MMP-8 in GCF and PISF increased with the severity of periodontitis and peri-implantitis. Enhanced levels of fibroblast-type MMP-8 in active form were detected only in severe CP GCF and PI PISF.

Conclusions: Peri-implantitis PISF contained higher collagenase-2 levels and activity than GCF from similar deep CP sites. GCF and PISF from severe CP and PI exhibited the highest activation of MMP-8 isoenzymes species (PMN and fibroblast-type).

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Alveolar Bone Loss / enzymology
  • Blotting, Western
  • Chronic Disease
  • Dental Implants*
  • Female
  • Fibroblasts / enzymology
  • Gingiva / enzymology
  • Gingival Crevicular Fluid / enzymology*
  • Gingival Hemorrhage / enzymology
  • Gingivitis / enzymology
  • Humans
  • Isoenzymes / analysis
  • Male
  • Matrix Metalloproteinase 8 / analysis*
  • Middle Aged
  • Neutrophils / enzymology
  • Periodontal Attachment Loss / enzymology
  • Periodontal Pocket / enzymology
  • Periodontitis / enzymology*
  • Periodontium / enzymology
  • Pilot Projects


  • Dental Implants
  • Isoenzymes
  • MMP8 protein, human
  • Matrix Metalloproteinase 8