Stabilizing effect of amphiphilic excipients on the freeze-thawing and freeze-drying of lactate dehydrogenase

K Izutsu; S Yoshioka; T Terao

doi:10.1002/bit.260431114

Stabilizing effect of amphiphilic excipients on the freeze-thawing and freeze-drying of lactate dehydrogenase

Biotechnol Bioeng. 1994 May;43(11):1102-7. doi: 10.1002/bit.260431114.

Authors

K Izutsu¹, S Yoshioka, T Terao

Affiliation

¹ National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, 158 Tokyo, Japan.

PMID: 18615522
DOI: 10.1002/bit.260431114

Abstract

The effects of amphiphilic excipients on the inactivation of lactate dehydrogenase (LDH) during freeze-thawing and freeze-drying were studied. Some amphiphilic excipients such as hydroxypropyl-beta-cyclodextrin (HP-beta-CD), CHAPS, polyethylene glycol (PEG) 3350, and sucrose fatty acid monoester prevented LDH inactivation during freeze-thawing and freeze-drying at a lower concentration than sugars and amino acids. Polyoxyethylene 9 lauryl ether and PEG 400 protected LDH during freeze-thawing but not during freeze-drying. The buffer concentration of the solution to be freeze-dried (10, 50, and 200 mM) affected the stabilizing effect of trehalose, but not that of HP-beta-CD. (c) 1994 John Wiley & Sons, Inc.