Specific requirements of MRFs for the expression of muscle specific microRNAs, miR-1, miR-206 and miR-133

Dev Biol. 2008 Sep 15;321(2):491-9. doi: 10.1016/j.ydbio.2008.06.019. Epub 2008 Jun 21.


The expression of three microRNAs, miR-1, miR-206 and miR-133 is restricted to skeletal myoblasts and cardiac tissue during embryo development and muscle cell differentiation, which suggests a regulation by muscle regulatory factors (MRFs). Here we show that inhibition of C2C12 muscle cell differentiation by FGFs, which interferes with the activity of MRFs, suppressed the expression of miR-1, miR-206 and miR-133. To further investigate the role of myogenic regulators (MRFs), Myf5, MyoD, Myogenin and MRF4 in the regulation of muscle specific microRNAs we performed gain and loss-of-function experiments in vivo, in chicken and mouse embryos. We found that directed expression of MRFs in the neural tube of chicken embryos induced ectopic expression of miR-1 and miR-206. Conversely, the lack of Myf5 but not of MyoD resulted in a loss of miR-1 and miR-206 expression. Taken together our results demonstrate differential requirements of distinct MRFs for the induction of microRNA gene expression during skeletal myogenesis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cell Differentiation / physiology
  • Cell Line
  • Chick Embryo
  • Fibroblast Growth Factors / metabolism
  • In Situ Hybridization
  • Mice
  • MicroRNAs / metabolism*
  • Molecular Sequence Data
  • Muscle Development / physiology*
  • Muscle, Skeletal / metabolism*
  • Myogenic Regulatory Factors / metabolism*
  • Neural Tube / metabolism*
  • Sequence Alignment


  • MicroRNAs
  • Myogenic Regulatory Factors
  • Fibroblast Growth Factors