Ammonium-dependent hydrogen peroxide production by mitochondria

FEBS Lett. 2008 Aug 6;582(18):2719-24. doi: 10.1016/j.febslet.2008.06.054. Epub 2008 Jul 11.


NADH-supported generation of H2O2 by permeabilized rat heart mitochondria was partially prevented by the specific complex I-directed inhibitor, NADH-OH, and was significantly stimulated by ammonium. Ammonium did not affect H2O2 production by complex I in coupled submitochondrial particles. The soluble mitochondrial matrix protein fraction catalyzed NADH-dependent H2O2 production, which was greatly (approximately 10-fold) stimulated by ammonium. We conclude that complex I is not the major contributor to mitochondrial superoxide (hydrogen peroxide) generation and that there are specific ammonium-sensitive NADH:oxygen oxidoreductase(s) in the mitochondrial matrix which are responsible for mitochondrial H2O2 production.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Catalysis
  • Cattle
  • Electron Transport Complex I / metabolism
  • Hydrogen Peroxide / metabolism*
  • Mitochondria, Heart / drug effects
  • Mitochondria, Heart / metabolism*
  • NAD / metabolism
  • Quaternary Ammonium Compounds / metabolism*
  • Quaternary Ammonium Compounds / pharmacology
  • Rats


  • Quaternary Ammonium Compounds
  • NAD
  • Hydrogen Peroxide
  • Electron Transport Complex I