Diagnostic utility of interferon-gamma-induced protein of 10 kDa (IP-10) in tuberculous pleurisy

Diagn Microbiol Infect Dis. 2008 Oct;62(2):186-92. doi: 10.1016/j.diagmicrobio.2008.05.011. Epub 2008 Jul 11.

Abstract

Tuberculous pleuritis (TP) is characterized by predominant Th1 immune response. We observed significantly high levels of interferon gamma (IFN-gamma) and chemokines such as IP-10, monokine induced by IFN-gamma (MIG), interleukin 8 (IL-8), monocyte chemotactic protein (MCP)-1, and macrophage inflammatory protein (MIP)-1alpha in tuberculous pleural effusions. In the current study, we evaluated the diagnostic utility of IFN-gamma-dependent chemokine especially IP-10. The receiver operating characteristics (ROC) curve analyses based on cytometric bead array values depicted high sensitivity only for IP-10 (76.3%) followed by IFN-gamma (73.7%). The ELISA test further confirmed the significantly high levels of IFN-gamma and IP-10 in TP. The ROC curve analysis again demonstrated high area under the curve (AUC) for IP-10 (0.966) than the referred diagnostic marker IFN-gamma (0.930). The better sensitivity (84.2% for IFN-gamma and 89.2% for IP-10) and equal specificity (95.7%) of IP-10 assay compared with IFN-gamma suggest that IP-10 is a potential diagnostic marker for evaluating TP.

Publication types

  • Evaluation Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Chemokine CXCL10* / metabolism
  • Female
  • Humans
  • Interferon-gamma / immunology
  • Interferon-gamma / metabolism
  • Male
  • Middle Aged
  • Pleural Effusion / immunology
  • Pleural Effusion / microbiology
  • ROC Curve
  • Sensitivity and Specificity
  • Tuberculosis, Pleural / diagnosis*
  • Tuberculosis, Pleural / immunology
  • Tuberculosis, Pleural / microbiology

Substances

  • CXCL10 protein, human
  • Chemokine CXCL10
  • Interferon-gamma