Seed defensins of barnyard grass Echinochloa crusgalli (L.) Beauv

Biochimie. Nov-Dec 2008;90(11-12):1667-73. doi: 10.1016/j.biochi.2008.06.007. Epub 2008 Jun 26.


From the annual weed barnyard grass Echinochloa crusgalli (L.) Beauv., two novel defensins Ec-AMP-D1 and Ec-AMP-D2 that differ by a single amino acid substitution were isolated by a combination of different chromatographic procedures. Both defensins were active against several phytopathogenic fungi and the oomycete Phytophthora infestans at micromolar concentrations. The Ec-AMP-D1 showed higher activity against the oomycete than Ec-AMP-D2. The comparison of the amino acid sequences of the antifungal E. crusgalli defensins with those of earlier characterized T. kiharae defensins [T.I. Odintsova, Ts.A. Egorov, A.Kh. Musolyamov, M.S. Odintsova, V.A. Pukhalsky, E.V. Grishin, Seed defensins from T. kiharae and related species: genome localization of defensin-encoding genes, Biochimie, 89 (2007) 605-612.] that were devoid of substantial antifungal activity point to the C-terminal region of the molecule as the main determinant of the antifungal activity of E. crusgalli defensins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acid Substitution
  • Anti-Infective Agents / isolation & purification*
  • Anti-Infective Agents / pharmacology*
  • Antifungal Agents / isolation & purification
  • Antifungal Agents / pharmacology
  • Defensins / genetics
  • Defensins / isolation & purification*
  • Defensins / pharmacology*
  • Echinochloa / chemistry*
  • Fungi / drug effects
  • Fungi / metabolism
  • Molecular Sequence Data
  • Phytophthora infestans / drug effects
  • Phytophthora infestans / metabolism
  • Seeds / chemistry
  • Sequence Alignment


  • Anti-Infective Agents
  • Antifungal Agents
  • Defensins