Functions of KLK4 and MMP-20 in dental enamel formation

Biol Chem. 2008 Jun;389(6):695-700. doi: 10.1515/BC.2008.080.

Abstract

Two proteases are secreted into the enamel matrix of developing teeth. The early protease is enamelysin (MMP-20). The late protease is kallikrein 4 (KLK4). Mutations in MMP20 and KLK4 both cause autosomal recessive amelogenesis imperfecta, a condition featuring soft, porous enamel containing residual protein. MMP-20 is secreted along with enamel proteins by secretory-stage ameloblasts. Enamel protein-cleavage products accumulate in the space between the crystal ribbons, helping to support them. MMP-20 steadily cleaves accumulated enamel proteins, so their concentration decreases with depth. KLK4 is secreted by transition- and maturation-stage ameloblasts. KLK4 aggressively degrades the retained organic matrix following the termination of enamel protein secretion. The principle functions of MMP-20 and KLK4 in dental enamel formation are to facilitate the orderly replacement of organic matrix with mineral, generating an enamel layer that is harder, less porous, and unstained by retained enamel proteins.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • Dental Enamel / cytology
  • Dental Enamel / enzymology
  • Dental Enamel / growth & development*
  • Dental Enamel / metabolism*
  • Humans
  • Kallikreins / metabolism*
  • Matrix Metalloproteinase 20 / metabolism*
  • Tooth / cytology
  • Tooth / enzymology
  • Tooth / growth & development
  • Tooth / metabolism

Substances

  • Kallikreins
  • kallikrein 4
  • Matrix Metalloproteinase 20