Native bacterial endophytes promote host growth in a species-specific manner; phytohormone manipulations do not result in common growth responses

PLoS One. 2008 Jul 16;3(7):e2702. doi: 10.1371/journal.pone.0002702.

Abstract

Background: All plants in nature harbor a diverse community of endophytic bacteria which can positively affect host plant growth. Changes in plant growth frequently reflect alterations in phytohormone homoeostasis by plant-growth-promoting (PGP) rhizobacteria which can decrease ethylene (ET) levels enzymatically by 1-aminocyclopropane-1-carboxylate (ACC) deaminase or produce indole acetic acid (IAA). Whether these common PGP mechanisms work similarly for different plant species has not been rigorously tested.

Methodology/principal findings: We isolated bacterial endophytes from field-grown Solanum nigrum; characterized PGP traits (ACC deaminase activity, IAA production, phosphate solubilization and seedling colonization); and determined their effects on their host, S. nigrum, as well as on another Solanaceous native plant, Nicotiana attenuata. In S. nigrum, a majority of isolates that promoted root growth were associated with ACC deaminase activity and IAA production. However, in N. attenuata, IAA but not ACC deaminase activity was associated with root growth. Inoculating N. attenuata and S. nigrum with known PGP bacteria from a culture collection (DSMZ) reinforced the conclusion that the PGP effects are not highly conserved.

Conclusions/significance: We conclude that natural endophytic bacteria with PGP traits do not have general and predictable effects on the growth and fitness of all host plants, although the underlying mechanisms are conserved.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / metabolism
  • Ecology
  • Ethylenes / chemistry
  • Gene Expression Regulation, Plant*
  • Microscopy, Confocal
  • Plant Growth Regulators / metabolism*
  • Plant Physiological Phenomena
  • Plant Roots
  • Plasmids / metabolism
  • RNA, Ribosomal, 16S / chemistry
  • Solanum nigrum / metabolism
  • Solanum nigrum / microbiology
  • Species Specificity
  • Symbiosis
  • Tobacco / microbiology

Substances

  • Ethylenes
  • Plant Growth Regulators
  • RNA, Ribosomal, 16S
  • ethylene