FISH for mapping single copy genes

Methods Mol Biol. 2008;422:31-49. doi: 10.1007/978-1-59745-581-7_3.

Abstract

During the past two decades fluorescent in-situ hybridization (FISH) has become a standard technique to directly localize, orient, and order genes in the genomes of a wide range of species. Despite the availability of a variety of probes, probe labeling and signal-detection systems, and advanced image analysis software, the core procedures used to carry out FISH remain the same. A detailed overview of these procedures, including target preparation (metaphase/interphase chromosomes and DNA fibers), probe labeling, in-situ hybridization, signal detection, and imaging, is here provided in a stepwise manner.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Chromosomes, Human / metabolism
  • DNA / metabolism
  • DNA Probes / metabolism
  • Gene Dosage / genetics*
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Interphase
  • Metaphase
  • Physical Chromosome Mapping*

Substances

  • DNA Probes
  • DNA