Elevated microsomal prostaglandin-E synthase-1 in Alzheimer's disease

Alzheimers Dement. 2008 Jan;4(1):6-13. doi: 10.1016/j.jalz.2007.10.015. Epub 2007 Dec 21.


Background: The proinflammatory prostaglandin E(2) (PGE(2)) fluctuates over time in the cerebrospinal fluid of patients with Alzheimer's disease (AD), but the cerebral distribution and expression patterns of microsomal prostaglandin-E synthase (mPGES)-1 have not been compared with those of normal human brains.

Methods: Middle frontal gyrus tissue from AD and age-matched control brains was analyzed by Western blot, immunofluorescence, and immunohistochemistry with mPGES-1-specific antibodies.

Results: Western blotting revealed that mPGES-1 expression was significantly elevated in AD tissue. Furthermore, immunofluorescence of mPGES-1 was observed in neurons, microglia, and endothelial cells of control and AD tissue. Although mPGES-1 was consistently present in astrocytes of control tissue, it was present in only some astrocytes of AD tissue. Immunohistochemical staining suggested that mPGES-1 was elevated in pyramidal neurons of AD tissue when compared with controls.

Conclusions: The results suggest that mPGES-1 is normally expressed constitutively in human neurons, microglia, astrocytes, and endothelial cells but is up-regulated in AD.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Aged
  • Aged, 80 and over
  • Alzheimer Disease / enzymology*
  • Astrocytes / enzymology
  • Blotting, Western
  • Brain / enzymology*
  • Endothelial Cells / enzymology
  • Female
  • Fluorescent Antibody Technique
  • Humans
  • Immunohistochemistry
  • Intramolecular Oxidoreductases / biosynthesis*
  • Male
  • Microglia / enzymology
  • Middle Aged
  • Neurons / enzymology
  • Prostaglandin-E Synthases
  • Up-Regulation


  • Intramolecular Oxidoreductases
  • PTGES protein, human
  • Prostaglandin-E Synthases