Liquid chromatography-tandem mass spectrometric quantification of the dehydration product of tetranor PGE-M, the major urinary metabolite of prostaglandin E(2) in human urine

J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Aug 1;871(1):72-7. doi: 10.1016/j.jchromb.2008.06.042. Epub 2008 Jul 1.


A LC-MS/MS method has been developed to analyze tetranor PGE-M, the major urinary metabolite of PGE(2), that involves the acid-catalyzed dehydration of tetranor PGE-M and its deuterated (d(6)) analog followed by LC-MS/MS measurement of the dehydrated tetranor PGE-M product (tetranor PGA-M). We also report a method for quantification of creatinine in urine by LC-MS/MS to normalize tetranor PGE-M concentrations with that of urinary creatinine. These methods were used to study the effect of aspirin on urinary tetranor PGE-M levels in healthy male volunteers. Aspirin did not affect urinary creatinine concentrations but decreased urinary tetranor PGE-M concentrations by approximately 44%.

Publication types

  • Validation Study

MeSH terms

  • Adult
  • Aspirin / pharmacology
  • Chromatography, Liquid / methods*
  • Creatinine / urine
  • Dinoprostone / metabolism*
  • Dinoprostone / urine
  • Drug Stability
  • Humans
  • Male
  • Prostaglandins / urine*
  • Reproducibility of Results
  • Tandem Mass Spectrometry / methods*


  • Prostaglandins
  • tetranor prostaglandin M
  • Creatinine
  • Dinoprostone
  • Aspirin