Intraocular pharmacokinetics of bevacizumab after a single intravitreal injection in humans

Am J Ophthalmol. 2008 Oct;146(4):508-12. doi: 10.1016/j.ajo.2008.05.036. Epub 2008 Jul 17.


Purpose: To investigate intraocular concentrations and pharmacokinetics of bevacizumab after a single intravitreal injection in humans.

Design: Prospective, noncomparative, interventional case series.

Methods: We included 30 nonvitrectomized eyes of 30 patients (age range, 43 to 93 years) diagnosed with clinically significant cataract and concurrent macular edema secondary to neovascular age-related macular degeneration, diabetic retinopathy, or retinal venous occlusion in the same eye. All patients received an intravitreal injection of 1.5 mg bevacizumab. Between one and 53 days after injection, an aqueous humor sample was obtained during elective cataract surgery. Concentrations of unbound bevacizumab in these samples were quantified by enzyme-linked immunosorbent assay.

Results: Concentration of bevacizumab in aqueous humor peaked on the first day after injection with a mean concentration (c(max)) of 33.3 microg/ml (range, 16.6 to 42.5 microg/ml) and subsequently declined in a monoexponential fashion. Nonlinear regression analysis determined an elimination half-time (t(1/2)) of 9.82 days (R(2) = 0.81). No significant differences between diagnosis subgroups were noted.

Conclusions: In human nonvitrectomized eyes, the aqueous half-life of 1.5 mg intravitreally injected bevacizumab is 9.82 days.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Angiogenesis Inhibitors / pharmacokinetics*
  • Antibodies, Monoclonal / pharmacokinetics*
  • Antibodies, Monoclonal, Humanized
  • Aqueous Humor / metabolism*
  • Bevacizumab
  • Cataract / complications
  • Cataract Extraction
  • Diabetic Retinopathy / complications
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Half-Life
  • Humans
  • Injections
  • Macular Degeneration / complications
  • Macular Edema / etiology
  • Male
  • Middle Aged
  • Prospective Studies
  • Retinal Vein Occlusion / complications
  • Vascular Endothelial Growth Factor A / antagonists & inhibitors
  • Vitreous Body


  • Angiogenesis Inhibitors
  • Antibodies, Monoclonal
  • Antibodies, Monoclonal, Humanized
  • Vascular Endothelial Growth Factor A
  • Bevacizumab