Royal jelly peptides inhibit lipid peroxidation in vitro and in vivo

J Nutr Sci Vitaminol (Tokyo). 2008 Jun;54(3):191-5. doi: 10.3177/jnsv.54.191.


Royal jelly peptides (RJPx) isolated from hydrolysates of water-soluble royal jelly proteins prepared with protease P exhibited significantly stronger hydroxyl radical-scavenging activity (p<0.001), and antioxidant activity against lipid peroxidation (LPO, p<0.001), than did water-soluble royal jelly protein (WSRJP) in vitro. We also investigated the in vivo antioxidant activity of RJPx against ferric nitrilotriacetate (Fe-NTA)-induced LPO. Male Wistar rats were divided into a control group (Group C), an Fe-NTA group (Group Fe), and an Fe-NTA with RJPx group (Group Fe+R). Rats in Group Fe+R were fed RJPx (2 g/kg body weight) daily for 5 wk. Fe-NTA (8 mg Fe/kg body weight) was then intraperitoneally injected, and serum lipid levels were examined 2 h later. Serum total cholesterol (TC) levels were lower (p<0.05) while low-density lipoprotein (LDL) and LPO were significantly higher (p<0.01) in Group Fe than in Group C. TC (p<0.05) and LPO levels (p<0.01) were lower in Group Fe+R than in Group Fe. Our data suggest that RJPx may inhibit LPO both in vitro and in vivo.

MeSH terms

  • Animals
  • Antioxidants / pharmacology
  • Carcinogens / toxicity
  • Cholesterol / blood
  • Fatty Acids / chemistry*
  • Ferric Compounds / toxicity
  • Free Radical Scavengers / pharmacology
  • Hydrolysis
  • Lipid Peroxidation / drug effects*
  • Lipids / blood
  • Male
  • Nitrilotriacetic Acid / analogs & derivatives
  • Nitrilotriacetic Acid / toxicity
  • Oxidative Stress / drug effects
  • Peptides / chemistry
  • Peptides / pharmacology*
  • Rats
  • Rats, Wistar
  • Time Factors


  • Antioxidants
  • Carcinogens
  • Fatty Acids
  • Ferric Compounds
  • Free Radical Scavengers
  • Lipids
  • Peptides
  • Cholesterol
  • Nitrilotriacetic Acid
  • royal jelly
  • ferric nitrilotriacetate