Bifidobacterium infantis suppresses proinflammatory interleukin-17 production in murine splenocytes and dextran sodium sulfate-induced intestinal inflammation

Int J Mol Med. 2008 Aug;22(2):181-5.


Interleukin (IL)-17 acts as a potent inflammatory cytokine, and IL-17-producing cells (Th17 cells) have received much attention. However, the involvement of commensal and/or probiotic bacteria in IL-17 production has not been evaluated. In this study, we examined the suppressive effects of five bacteria species on IL-17 production in vitro and ex vivo. Among the five species studied, Bifidobacterium infantis inhibited IL-17 production but enhanced IL-27 production most potently in TGF-beta plus IL-6-stimulated murine splenocytes. B. infantis also inhibited IL-17 and eotaxin production from a dextran sodium sulfate-treated colon organ culture. The induction of IL-10 by B. infantis was observed both in the splenocytes and in the colon culture and was assumed, to a certain extent, to be important for suppressing IL-17 production. These findings suggest a novel immunomodulatory function of commensal bifidobacteria and further imply that these bacteria may be useful in the treatment of Th17-mediated diseases.

MeSH terms

  • Animals
  • Bifidobacterium / immunology*
  • Cells, Cultured
  • Chemokine CCL11 / metabolism
  • Chemokines / metabolism
  • Dextran Sulfate / immunology*
  • Female
  • Humans
  • Inflammation / immunology*
  • Interleukin-10 / immunology
  • Interleukin-17 / immunology*
  • Interleukins / immunology
  • Intestines / anatomy & histology
  • Intestines / immunology*
  • Intestines / pathology*
  • Mice
  • Mice, Inbred BALB C
  • Probiotics
  • Spleen / cytology*
  • Spleen / immunology
  • Tissue Culture Techniques


  • Chemokine CCL11
  • Chemokines
  • Il27 protein, mouse
  • Interleukin-17
  • Interleukins
  • Interleukin-10
  • Dextran Sulfate