Transplantation of meniscus regenerated by tissue engineering with a scaffold derived from a rat meniscus and mesenchymal stromal cells derived from rat bone marrow

Artif Organs. 2008 Jul;32(7):519-24. doi: 10.1111/j.1525-1594.2008.00580.x.

Abstract

The purpose of this study was to assess transplantation of regenerated menisci using scaffolds from normal allogeneic menisci and bone-marrow-derived mesenchymal stromal cells (BM-MSCs) of rats. We reported that scaffolds derived from normal menisci seeded with BM-MSCs in vitro could form meniscal tissues within 4 weeks. Then, we hypothesized that our tissues could be more beneficial than allogeneic menisci regarding early maturation and chondroprotective effect. Bone marrow was aspirated from enhanced green fluorescent protein transgenic rats. BM-MSCs were isolated and seeded onto scaffolds which were prepared from Sprague-Dawley rat menisci. After 4 weeks in coculture, the tissues were transplanted to the defect of menisci. Repopulation of BM-MSCs and expression of extracellular matrices were observed in the transplanted tissues at 4 weeks after surgery. At 8 weeks, articular cartilage in the cell-free group was more damaged compared to that in the cell-seeded group or the meniscectomy group.

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Bone Marrow Cells / cytology*
  • Bone Marrow Cells / physiology*
  • Cells, Cultured
  • Extracellular Matrix / physiology
  • Green Fluorescent Proteins / metabolism
  • Guided Tissue Regeneration*
  • Male
  • Menisci, Tibial / physiology
  • Menisci, Tibial / transplantation*
  • Mesenchymal Stem Cells / physiology*
  • Rats
  • Rats, Sprague-Dawley
  • Regeneration
  • Stromal Cells
  • Tissue Culture Techniques
  • Tissue Engineering*
  • Tissue Scaffolds*

Substances

  • Green Fluorescent Proteins