Comparison of sequence diversity in several cytoplasmic polyhedrosis viruses

M S Galinski; V H Stanik; G F Rohrmann; G S Beaudreau

doi:10.1016/0042-6822(83)90091-0

Comparison of sequence diversity in several cytoplasmic polyhedrosis viruses

Virology. 1983 Oct 30;130(2):372-80. doi: 10.1016/0042-6822(83)90091-0.

Authors

M S Galinski¹, V H Stanik, G F Rohrmann, G S Beaudreau

Affiliation

¹ Department of Agricultural Chemistry, Oregon State University, Corvallis, Oregon 97331, USA.

PMID: 18639153
DOI: 10.1016/0042-6822(83)90091-0

Abstract

Sequence homology among several different cytoplasmic polyhedrosis virus (CPV) types and the human reovirus (type 1) was examined by Northern blot analysis and S, nuclease analysis, using random-primed cDNA probes synthesized from total genomic RNA. The results show no homology among the CPV type 1, Bombyx mori CPV, type 5, Orgyia pseudotsugata CPV, type 8, Manduca sexta CPV and the human reovirus (type 1). However, there was significant homology among three type 5 CPVs, O. pseudotsugata CPV, Euxoa scandens CPV, and Heliothis armigera CPV. The O. pseudotsugata CPV and E. scandens CPV were 43-44% homologous while each was 6-13% homologous with the H. armigera CPVusing stringent conditions of hybridization.