Ganoderma lucidum polysaccharides can induce human monocytic leukemia cells into dendritic cells with immuno-stimulatory function

J Hematol Oncol. 2008 Jul 21;1:9. doi: 10.1186/1756-8722-1-9.

Abstract

Background: Previous studies demonstrated Ganoderma lucidum polysaccharides (GL-PS), a form of bioactive beta-glucan can stimulate the maturation of monocyte-derived dendritic cells (DC). The question of how leukemic cells especially in monocytic lineage respond to GL-PS stimuli remains unclear.

Results: In this study, we used in vitro culture model with leukemic monocytic cell-lines THP-1 and U937 as monocytic effectors cells for proliferation responses and DCs induction. We treated the THP-1 and U937 cells with purified GL-PS (100 microg/mL) or GL-PS with GM-CSF/IL-4. GL-PS alone induced proliferative response on both THP-1 and U937 cells but only THP-1 transformed into typical DC morphology when stimulated with GL-PS plus GM-CSF/IL-4. The transformed THP-1 DCs had significant increase expression of HLA-DR, CD40, CD80 and CD86 though not as high as the extent of normal monocyte-derived DCs. They had similar antigen-uptake ability as the normal monocyte-derived DCs positive control. However, their potency in inducing allogeneic T cell proliferation was also less than that of normal monocyte-derived DCs.

Conclusion: Our findings suggested that GL-PS could induce selected monocytic leukemic cell differentiation into DCs with immuno-stimulatory function. The possible clinical impact of using this commonly used medicinal mushroom in patients with monocytic leukemia (AML-M4 and M5) deserved further investigation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / pharmacology*
  • Cell Line, Tumor
  • Cell Proliferation
  • Dendritic Cells / cytology
  • Dendritic Cells / immunology*
  • Endocytosis
  • Granulocyte-Macrophage Colony-Stimulating Factor / immunology
  • Humans
  • Interleukin-10 / immunology
  • Interleukin-12 / immunology
  • Interleukin-4 / immunology
  • Leukemia / immunology*
  • Monocytes / cytology
  • Monocytes / immunology*
  • Polysaccharides / immunology*
  • Polysaccharides / isolation & purification
  • Reishi / chemistry*
  • T-Lymphocytes / cytology

Substances

  • Adjuvants, Immunologic
  • Polysaccharides
  • Interleukin-10
  • Interleukin-12
  • Interleukin-4
  • Granulocyte-Macrophage Colony-Stimulating Factor