Abstract
The ERG3 gene from Saccharomyces cerevisiae has been cloned by complementation of an erg3-2 mutation. ERG3 is the putative gene encoding the C-5 sterol desaturase required for ergosterol biosynthesis. The functional gene has been localized on a 2.5-kb HindIII-BamHI fragment containing an open reading frame comprising 365 amino acids. Gene disruption resulting from a deletion/substitution demonstrates that ERG3 is not essential for cell viability or the sparking function.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Southern
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Cloning, Molecular
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Ergosterol / biosynthesis*
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Frameshift Mutation
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Molecular Sequence Data
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Mutagenesis, Insertional
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Oxidoreductases / chemistry
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Oxidoreductases / genetics*
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Restriction Mapping
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics*
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Sterols / metabolism*
Substances
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Sterols
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Oxidoreductases
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sterol delta-5 desaturase
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Ergosterol
Associated data
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GENBANK/D90323
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GENBANK/D90324
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GENBANK/D90325
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GENBANK/D90326
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GENBANK/D90327
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GENBANK/M61155
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GENBANK/S46162
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GENBANK/S49891
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GENBANK/S53006
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GENBANK/X55890