Isolation of two genes encoding putative protein kinases regulated during Dictyostelium discoideum development

Gene. 1991 Jun 15;102(1):57-65. doi: 10.1016/0378-1119(91)90538-m.

Abstract

Two Dictyostelium discoideum protein kinase(PK)-encoding cDNAs (Dd PK1 and Dd PK2) have been isolated by hybridization with an oligodeoxyribonucleotide derived from a highly conserved region of eukaryotic PKs. The two nucleotide (nt) sequences encode new putative serine/threonine-specific PKs. Dd PK1 is a partial cDNA covering the entire catalytic domain. The derived amino acid (aa) sequence is about 30% identical to both cAMP-dependent protein kinase (cAPK) and protein kinase C. The Dd PK2 sequence was extended through the isolation of a genomic fragment encoding a complete putative protein. A single intron is present, as deduced from sequence comparison with the cDNA. The catalytic domain appears more closely related to the catalytic subunit of cAPK (54% sequence identity). However, our nt sequence potentially codes for a much larger protein (648 vs. about 350 aa for most cAPKs) with a N-terminal half containing long homopolymers of threonines, glutamines and asparagines. Similar repeats occur at the C terminus of Dd PK1, Dd PK1 is expressed in vegetatively growing cells and during development. Dd PK1 RNA decreases after 6 h of starvation to re-accumulate once the cells have aggregated. Dd PK2 transcripts, present at a low amount in growing cells, rise upon starvation. A switch to a shorter form of transcripts occurs between 3 and 6 h into development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • Dictyostelium / enzymology
  • Dictyostelium / genetics*
  • Dictyostelium / growth & development
  • Gene Expression Regulation, Fungal / physiology*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Protein Kinases / genetics*
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid

Substances

  • Protein Kinases

Associated data

  • GENBANK/D90323
  • GENBANK/D90324
  • GENBANK/D90325
  • GENBANK/D90326
  • GENBANK/D90327
  • GENBANK/M38703
  • GENBANK/M38704
  • GENBANK/M38794
  • GENBANK/M61904
  • GENBANK/S46365
  • GENBANK/S49891
  • GENBANK/X55890