Identification of the coat protein gene of bean golden mosaic geminivirus

Intervirology. 1991;32(4):258-61. doi: 10.1159/000150208.

Abstract

An open-reading frame designated 1R1, on DNA component 1 of bean golden mosaic geminivirus (BGMV), has been identified as the coat protein gene. A DraI restriction fragment of BGMV DNA1 that includes 1R1 was inserted into the SmaI pKK223-3 expression vector. The 32 kD protein expressed in Escherichia coli cells reacted with antibodies to the BGMV capsid polypeptide and behaved identically to purified capsid protein in western blots.

MeSH terms

  • Animals
  • Capsid / genetics*
  • DNA, Viral / analysis
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / genetics
  • Gene Expression
  • Genetic Vectors
  • Immunoblotting
  • Mosaic Viruses / genetics*
  • Open Reading Frames / genetics
  • Rabbits
  • Transfection

Substances

  • DNA, Viral