Abstract
A peptide-based 2-D liquid phase fractionation (PF2D) system was used in a quantitative proteomic analysis of hepatocellular carcinoma. 2-D liquid maps of peptide specimens showed better resolution than those of proteins, leading to the identification of differentially expressed proteins. Peptide-based PF2D gave well-matched theoretical and experimental pI values and was proven to be a very efficient and versatile analytical tool for both large-scale profiling and quantification of phosphoproteins in disease biomarker discovery.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Alkaline Phosphatase / metabolism
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Biomarkers / analysis*
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Biomarkers / blood
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Carcinoma, Hepatocellular / blood
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Carcinoma, Hepatocellular / diagnosis*
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Carcinoma, Hepatocellular / metabolism
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Chemical Fractionation / methods*
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Chromatography, Liquid
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Electrophoresis, Gel, Two-Dimensional
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Humans
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Male
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Peptides / chemistry
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Peptides / metabolism*
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Proteomics / methods*
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Reproducibility of Results
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Tandem Mass Spectrometry
Substances
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Biomarkers
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Peptides
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Alkaline Phosphatase