Catabolite repression by galactose in overexpressed GAL4 strains of Saccharomyces cerevisiae

J Gen Microbiol. 1991 May;137(5):1039-44. doi: 10.1099/00221287-137-5-1039.


Catabolite repression by galactose was investigated in several strains of Saccharomyces cerevisiae grown on different carbon sources. Galactose repressed as much as glucose; raffinose was less effective. Full derepression was achieved with lactate. The functions tested were L-lactate ferricytochrome c oxidoreductase, NAD-glutamate dehydrogenase, and respiration. Galactose repression was observed only in the GAL4 but not in the gal4 strain. The presence of multiple copies of the GAL4 gene enhanced the repression by galactose. Different alleles of the GAL4 gene and the copy number did not affect glucose repression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • DNA-Binding Proteins
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Galactose / metabolism*
  • Galactose / pharmacology
  • Gene Expression
  • Genes, Fungal
  • Glutamate Dehydrogenase / metabolism
  • L-Lactate Dehydrogenase (Cytochrome)
  • L-Lactate Dehydrogenase / metabolism
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins*
  • Species Specificity
  • Transcription Factors / genetics
  • Transcription Factors / metabolism


  • DNA-Binding Proteins
  • Fungal Proteins
  • GAL4 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • L-Lactate Dehydrogenase
  • L-Lactate Dehydrogenase (Cytochrome)
  • Glutamate Dehydrogenase
  • Galactose