Transcriptomic profiles of Drosophila melanogaster third instar larval midgut and responses to oxidative stress

Insect Mol Biol. 2008 Aug;17(4):325-39. doi: 10.1111/j.1365-2583.2008.00808.x.


Oligoarray analysis was used to determine the number and nature of genes expressed in third instar Drosophila melanogaster larval midguts. The majority of transcripts were associated with protein synthesis and metabolism. Serine proteases were the main proteolytic enzymes detected. Some 40% of the cytochrome P450 genes and 74% of the glutathione S transferases (GSTs) in the genome of D. melanogaster were observed to be expressed in the midgut by oligoarray analysis. We also identified potential transcription factor binding motifs (TFBMs) of P450s, GSTs and carboxylesterases. Many of the midgut-expressed GST genes contained candidate TFBMs homologous to TFBMs in mammals that have been associated with responses to oxidative stress. We also investigated the response of GSTs in the midgut to dietary H2O2, which showed a dosage-based differential response.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Carboxylic Ester Hydrolases / genetics
  • Carboxylic Ester Hydrolases / metabolism
  • Cytochrome P-450 Enzyme System / genetics
  • Cytochrome P-450 Enzyme System / metabolism
  • Drosophila melanogaster / drug effects
  • Drosophila melanogaster / genetics
  • Drosophila melanogaster / growth & development
  • Drosophila melanogaster / metabolism*
  • Expressed Sequence Tags
  • Gastrointestinal Tract / growth & development
  • Gastrointestinal Tract / metabolism*
  • Gene Expression Profiling*
  • Gene Expression Regulation / drug effects
  • Genome
  • Glutathione Transferase / genetics
  • Glutathione Transferase / metabolism
  • Hydrogen Peroxide / pharmacology
  • Larva / drug effects
  • Larva / genetics
  • Larva / growth & development
  • Larva / metabolism
  • Oligonucleotide Array Sequence Analysis
  • Oxidative Stress / drug effects
  • Serine Endopeptidases / genetics
  • Serine Endopeptidases / metabolism


  • Cytochrome P-450 Enzyme System
  • Hydrogen Peroxide
  • Glutathione Transferase
  • Carboxylic Ester Hydrolases
  • Serine Endopeptidases