[Apoptosis of HL-60 cells induced by crystal proteins from Bacillus thuringiensis Bt9875]

Wei Sheng Wu Xue Bao. 2008 May;48(5):690-4.
[Article in Chinese]

Abstract

Objective: To study the effect of Bt9875 crystal protein treated with proteinase K on human cancer cells, HL-60.

Methods: We used the methods of Thiazolyl Blue Tetrazolium Bromide, fluorescence microscopy examination, agarose gel electrophores and flow cytometry to detect the growth inhibition rate and apoptosis characters of the HL-60 cells that were treated with different concentration of Bt9875 crystal protein.

Results: Bt9875 crystal protein inhibited the growth of HL-60 cells evidently in a dose-dependent manner, with minimal effects on normal human peripheral blood mononuclear cells (PBMCs). The nuclei of HL-60 cells showed the characteristics of apoptosis. The analysis by flow cytometry indicated that the apoptosis rate of HL-60 cells was 52% after treatment with Bt9875 crystal protein (100 microg/mL). DNA analyzed by agarose gel electrophoresis showed "ladder" pattern.

Conclusion: Bt9875 crystal protein could inhibit the growth of HL-60 and induced its apoptosis, which provided a foundation for use of Bt9875 crystal protein to cure the acute myeloid leukemia.

Publication types

  • English Abstract

MeSH terms

  • Apoptosis / drug effects*
  • Bacillus thuringiensis / chemistry*
  • Bacillus thuringiensis / classification
  • Bacillus thuringiensis / isolation & purification
  • Bacterial Proteins / isolation & purification
  • Bacterial Proteins / pharmacology*
  • Cell Nucleus / drug effects
  • Cell Nucleus / metabolism
  • DNA Fragmentation / drug effects
  • Dose-Response Relationship, Drug
  • Electrophoresis, Polyacrylamide Gel
  • Endotoxins / isolation & purification
  • Endotoxins / pharmacology*
  • Flow Cytometry
  • HL-60 Cells
  • Hemolysin Proteins / isolation & purification
  • Hemolysin Proteins / pharmacology*
  • Humans
  • Microscopy, Fluorescence

Substances

  • Bacterial Proteins
  • Endotoxins
  • Hemolysin Proteins
  • insecticidal crystal protein, Bacillus Thuringiensis