[Changes of proteomic spectra of total flavones of Hippophae rhamnoides on myocardial protection]

Zhongguo Zhong Yao Za Zhi. 2008 May;33(9):1060-3.
[Article in Chinese]

Abstract

Objective: To study the mechanisms of the anti-myocardial ischemia of total flavones of Hippophae rhamnoides (TFH) at the level of proteome.

Method: Surface enhanced laser desorption/ionization (SELDI) mass spectrometry with protein chip IMAC3, SAX2 and NP20 was performed to compare the differentially expressed protein in myocardial ischemia in the TFH-treated groups with the 0.9% sodium chloride groups. Protein chips were examined in PBS II - C protein chip reader (ciphergen ciosystem inc) and the protein profiling was analyzed by Proteinchip Software 3. 0. 2.

Result: The revealed six peaks had significant difference between the TFH-treated groups and the control groups, one of which were up-regulated in the TFH-treated groups, and the other were down-regulated. And in these six distinct proteins, there were four proteins on the IMAC3 chips and one protein on the SAX2 chips.

Conclusion: The TFH could prevent the myocardium from ischemia via regulating expression of different proteins.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Drugs, Chinese Herbal / pharmacology*
  • Female
  • Flavones / chemistry
  • Flavones / pharmacology*
  • Heart / drug effects*
  • Hippophae / chemistry*
  • Male
  • Myocardial Ischemia / prevention & control
  • Proteomics / methods*
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Drugs, Chinese Herbal
  • Flavones