LEDGFp52 controls rat retinal ganglion cell neurite growth in culture and regulates specific neuronal growth-associated genes and protein production

J Int Med Res. Jul-Aug 2008;36(4):815-29. doi: 10.1177/147323000803600425.

Abstract

We investigated the regulation of primary neurite growth and expression of specific growth-associated genes by lens epithelium-derived growth factor (LEDGF) in rat retinal ganglion cells (RGCs). A pAd-LEDGFp52 adenovirus vector and a siRNA-LEDGFp52 eucaryotic expression vector were transfected into cultured RGCs. Transfection with pAd-LEDGFp52 significantly increased the number of neurites and their lengths compared with untransfected control RGCs. The expression of growth associated protein 43 (GAP43), microtubule-associated protein 2 (MAP2), and low-molecular-weight neurofilament (NF-L) genes and proteins were also significantly up-regulated. In contrast, the introduction of siRNA-LEDGFp52 significantly decreased the number and length of neurites, and significantly down-regulated the expression GAP43, NF-L and MAP2 genes and proteins compared with controls. Our findings suggest that LEDGFp52 might act as a dendritic arborization gene as well as an axonal elongation gene in RGCs and that it might be beneficial to the functional recovery of regenerating RGCs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / genetics
  • Adaptor Proteins, Signal Transducing / metabolism*
  • Adenoviridae / genetics
  • Adenoviridae / metabolism
  • Animals
  • Cell Culture Techniques
  • Cell Shape
  • Cells, Cultured
  • GAP-43 Protein / metabolism
  • Genetic Vectors
  • Microtubule-Associated Proteins / metabolism
  • Nerve Tissue Proteins* / genetics
  • Nerve Tissue Proteins* / metabolism
  • Neurites / metabolism*
  • Neurites / ultrastructure
  • Neurofilament Proteins / metabolism
  • Protein Isoforms / genetics
  • Protein Isoforms / metabolism
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / metabolism
  • Rats
  • Rats, Long-Evans
  • Retinal Ganglion Cells / cytology*
  • Retinal Ganglion Cells / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*

Substances

  • Adaptor Proteins, Signal Transducing
  • GAP-43 Protein
  • Microtubule-Associated Proteins
  • Nerve Tissue Proteins
  • Neurofilament Proteins
  • Protein Isoforms
  • Psip1 protein, rat
  • RNA, Small Interfering
  • Transcription Factors
  • neurofilament protein L