Secretome analysis of Phanerochaete chrysosporium strain CIRM-BRFM41 grown on softwood

Appl Microbiol Biotechnol. 2008 Sep;80(4):719-33. doi: 10.1007/s00253-008-1596-x. Epub 2008 Jul 25.


Proteomic analysis was performed to determine and differentiate the composition of the secretomes of Phanerochaete chrysosporium CIRM-BRFM41, a peroxidase hypersecretory strain grown under ligninolytic conditions and on softwood chips under biopulping conditions. Extracellular proteins from both cultures were analyzed by bidimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry. A total of 37 spots were identified. The secretome in liquid synthetic medium comprised mainly peroxidases, while several wood-degrading enzymes and enzymes involved in fungal metabolism were detected in biopulping cultures on softwood. This prompted an analysis of the impact of secretome modulation in the presence of softwood chips. Biotreated wood was submitted to kraft cooking and chemical bleaching using chlorine dioxide. The fungal pre-treatment led to a significant increase in pulp yield and a better bleachability of the pulp. This bleachability improvement could be explained by the production of specific lignocellulose-degrading enzymes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Electrophoresis, Gel, Two-Dimensional
  • Extracellular Space / chemistry
  • Extracellular Space / genetics
  • Extracellular Space / metabolism*
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • Industrial Microbiology*
  • Lignin / chemistry
  • Lignin / metabolism*
  • Molecular Sequence Data
  • Phanerochaete / chemistry
  • Phanerochaete / genetics
  • Phanerochaete / metabolism*
  • Protein Transport
  • Proteomics*
  • Wood / chemistry
  • Wood / metabolism*


  • Fungal Proteins
  • Lignin