Purification and mass spectrometric analysis of the kappa opioid receptor

Brain Res. 2008 Sep 16;1230:13-26. doi: 10.1016/j.brainres.2008.06.121. Epub 2008 Jul 12.

Abstract

A clonal human embryonic kidney (HEK) 293 cell line was established that stably expressed the rat kappa-opioid receptor (rKOR) with a FLAG epitope at the amino terminus. The Kd for [3H]diprenorphine was 1.1+/-0.2 nM, and the Bmax was 2.6+/-0.4 pmol/mg. Dynorphin A (1-13), U69,593 and naloxone competitively inhibited [3H]diprenorphine binding with Ki values of 2.0, 18 and 18 nM, respectively, in good agreement with previously reported affinities for the unmodified receptor. U69,593 stimulated [35S]GTPgammaS binding in a concentration-dependent manner and caused phosphorylation of mitogen-activated protein (MAP) kinase, indicating that the activated epitope-tagged receptor triggered appropriate signaling pathways. Immunoblot analysis demonstrated that two immunoreactive receptor species with apparent molecular masses of 42 and 52 kDa were expressed. Previous studies indicated that the 42 kDa protein was localized intracellularly and was a precursor of the 52 kDa receptor, which was present at the cell surface. rKOR was extracted from transfected HEK 293 cell membranes with n-dodecyl-beta-D-maltopyranoside. Sequential use of wheat germ agglutinin chromatography, Sephacryl S300 gel filtration chromatography, anti-FLAG immunoaffinity chromatography and SDS/PAGE permitted purification of the 52 kDa receptor. MALDI-TOF mass spectrometry was used to identify peptides derived from rKOR following sequential in-gel digestion with trypsin and cyanogen bromide. Eighteen rKOR peptides were detected, corresponding to 27.1% coverage of the receptor. Precursor-selective MS/MS confirmed the identity of most of these peptides. In addition, we have identified heat shock protein 70 (HSP70) as a rKOR-interacting protein.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Blotting, Western
  • Cell Line
  • Chromatography, Affinity
  • Chromatography, Agarose
  • Chromatography, Gel
  • Electrophoresis, Polyacrylamide Gel
  • Guanosine 5'-O-(3-Thiotriphosphate) / metabolism
  • HSP70 Heat-Shock Proteins / analysis
  • Humans
  • Immunochemistry
  • Immunoprecipitation
  • Mass Spectrometry
  • Membranes / chemistry
  • Membranes / metabolism
  • Protein Hydrolysates / chemistry
  • Radioligand Assay
  • Receptors, Opioid, kappa / chemistry
  • Receptors, Opioid, kappa / isolation & purification*
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / isolation & purification
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Wheat Germ Agglutinins / chemistry

Substances

  • HSP70 Heat-Shock Proteins
  • Protein Hydrolysates
  • Receptors, Opioid, kappa
  • Recombinant Proteins
  • Wheat Germ Agglutinins
  • Guanosine 5'-O-(3-Thiotriphosphate)